Research work/Study for Nobel Prize in Physiology or Medicine

For ADULT patients:

(a) 'Silence' HLA gene on donor organ, then proceed with organ transplantation: Transfect macrophage, L929, or HEK293 cells with siRNA (silencing RNA) mixture consisting of HLA-A, HLA-B, HLA-C, HLA-DPA1, HLA-DPB1, HLA-DQA1, HLA-DQB1, HLA-DRA, HLA-DRB1 (to be developed)(1,2,3,4,5,6,7). Grow cells till they reach 90% confluence, isolate siRNA from cells, add siRNA mix to 'UW' solution (8,9,10) at concentration of 100 micrograms/ml. Place donor organ in this solution @4 degrees celsius for 48 hours (8). Proceed to organ transplantation, step (b).Note: Due to the current price of siRNA, option (a) is therapeutically VERY EXPENSIVE and NOT FEASIBLE unless the patient is VERY RICH

(b) If step (a) fails/not economically feasible, proceed to Thymectomy first; then perform organ transplantation

For MINOR patients (kidney, liver):

(c) Organ transplantation

(d) Sirolimus monotherapy (11,12)

References:

(1) http://datasheets.scbt.com/sc-43929.pdf

(2) http://datasheets.scbt.com/sc-37113.pdf

(3) http://datasheets.scbt.com/sc-105525.pdf

(4) http://datasheets.scbt.com/sc-42917.pdf

(5) http://datasheets.scbt.com/sc-42922.pdf

(6) http://datasheets.scbt.com/sc-42908.pdf

(7) http://datasheets.scbt.com/sc-42918.pdf

(8) http://circ.ahajournals.org/content/120/12/1099.full

(9) http://www.ncbi.nlm.nih.gov/pubmed/1689516

(10) http://en.wikipedia.org/wiki/Viaspan

(11) http://www.clinicaltrials.gov/ct/show/NCT00768729

(12) http://www.ncbi.nlm.nih.gov/pubmed/17692656
Additional details have been emailed to the Nobel Prize Foundation for review and available upon request.